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Culture of Animal Cells 8/E
ÆǸŰ¡°Ý  : 65,000¿ø
Àû¸³±Ý  : 1,950Á¡
ÃâÆǻ砠: Wiley
ÀúÀÚ  : Freshney
¹ßÇàÀÏ  : 2021³â 6¿ù
ÆÇÇü  : 215*275
ÆäÀÌÁö ¼ö  : 832¸é
ISBN  : 9781119513018
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THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES

Freshney¡¯s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more.

The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text:

 

Part I UNDERSTANDING CELL CULTURE

Chapter 1 Introduction

Chapter 2 Biology of Cultured Cells

Chapter 3 Origin and Evolution of Cultured Cells

Chapter 4 Laboratory Design and Layout


Part  II LABORATORY AND REGULATORY REQUIREMENTS

Chapter 5 Equipment and Materials

Chapter 6 Safety and Bioethics

Chapter 7 Reproducibility and Good Cell Culture Practice


Part III MEDIUM AND SUBSTRATE REQUIREMENTS

Chapter 8 Culture Vessels and Substrates

Chapter 9 Defined Media and Supplements

Chapter 10 Serum-free Media

Chapter 11 Preparation and Sterilization


Part IV HANDLING CULTURES

Chapter 12 Aseptic Technique

Chapter 13 Primary Culture

Chapter 14 Subculture and Cell Lines

Chapter 15 Cryopreservation and Banking

Part V VALIDATION AND CHARACTERIZATION

Chapter 16 Microbial Contamination

Chapter 17 Cell Line Misidentification and Authenticatio

Chapter 18 Cell Line Characterization

Chapter 19 Quantitation and Growth KineticsChapter 


Part VI PHYSICAL AND GENETIC MANIPULATION

Chapter 20 Cell Cloning and Selection

Chapter 21 Cell Separation and Sorting

Chapter 22 Genetic Modification and Immortalization


Part VII STEM CELLS AND DIFFERENTIATED CELLS

Chapter 23 Culture of Stem Cells

Chapter 24 Culture of Specific Cell Types

Chapter 25 Culture of Tumor Cells

Chapter 26 Differentiation


Part VIII MODEL ENVIRONMENTS AND APPLICATIONS

Chapter 27 Three-dimensional Culture

Chapter 28 Scale-up and Automation

Chapter 29 Toxicity Testing


Part IX TEACHING AND TROUBLESHOOTING

Chapter 30 Training

Chapter 31 Problem Solving

Chapter 32 In Conclusion

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