Introduction: Laboratory Operations and Safety: Hand Washing.

PART I.  GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE.

1. Laboratory Safety: Introduction to the Microscope.

2. Transfer and Isolation Techniques.

Microbes in the Environment.

PART II. MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS.

3. Cultural and Cellular Morphology.

4. Bacterial Growth.

5. Gram Stain.

Acid-Fast Stain.

6. Endospore Stain.

Capsule Stain.

Hanging Drop Technique.

7. Fungi.

8. Viruses—Visualization and Enumeration.

9. Parasitology.

PART III.  MICROBIAL CONTROL AND BIOCHEMISTRY.

10. Microbial Sensitivity Testing:

Ultraviolet Light Sensitivity.

Heat Sensitivity.

Chemical Sensitivity.

Antibiotic Sensitivity:

Kirby-Bauer Technique.

11. Bacterial Biochemistry.

12. Gas Requirements of Microorganisms.

13. Specialized Media.

PART IV.  MEDICAL MICROBIOLOGY.

14. Genetics.

15. Epidemiology.

16. Speciman-Handling Protocols.

17. Specific Laboratory Tests.

18. Serology.

19. Identification of Enteric Pathogens: Traditional Methods.

20. Identification of Enteric Pathogens: Rapid Identification Methods.

21. Identification of a Bacterial Unknown: The Gram-Negative Unknown.

22. Identification of a Bacterial Unknown: The Gram-Positive Cocci.

PART V.  FOOD AND ENVIRONMENTAL MICROBIOLOGY.

23. Identification and Quantification of Microbial Numbers in a Water Sample.

24. Identification of Microbes in Beef and Poultry and the Quantification of Microbial Numbers.

25. Soil Microbiology.

Photographic Atlas.

26. Microbial Ecology.

27. Biofilms.

Bibliography.

Appendix 1.

Appendix 2.

Appendix 3.

Credits.

Index.